(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 161 PubMed citations. Related Posts. Company Promega; Price Supplier Page View Company Product Page; Catalog Number A3610; Quantity 20 reactions; Vector Name pGEM®-T Vector; Applications • Cloning PCR products. • Construction of unidirectional nested deletions with the Erase-a-Base® System. Bioz Stars score: 97/100, based on 572 PubMed citations. Instructions for Use of Product(s) E1910, E1960. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 177 PubMed citations. Luminometer and Microplate Reader Selection Guide. • Production of ssDNA. Pgemt Promega Polylinker, supplied by Promega, used in various techniques. Promega’s pGEM-T vector systems are very dependable and versatile tools to routinely clone fragments of DNA into reliable plasmids. Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. Contact Technical Services. • Blue/white screening for recombinants. Quick Protocols. The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends. 1/21 Apoptosis Assays ProductSize Cat.# Caspase-Glo® 3/7 Assay System 2.5ml G8090 Caspase-Glo® 3/7 3D Assay System 10ml G8981 RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay 100 assays JA1011 Additional … These single-nucleotide overhangs anneal to the 3´‑thymidine overhangs of T-cloning vectors such as the Promega pGEM®-T (Cat.# A3600) and pGEM®-T Easy Vectors (Cat.# A1360) and the Invitrogen TOPO® TA Cloning Kit with pCR®2.1-TOPO® Vector (Invitrogen Cat.# K4500-01). Die von Ihnen gewünschte Seite kann nicht gefunden werden, oder ist momentan nicht erreichbar. • Blue/white screening for recombinants. • In vitro transcription from dual-opposed promoters. Clark, J.M. Theoretically, smaller inserts will be cloned more efficiently than longer inserts. Pgemt Easy Vector, supplied by Promega, used in various techniques. “Reporter Gene Assay Kits Market Scenario 2020-2027:. Pgemt Vector, supplied by Promega, used in various techniques. Literature # TM040. プロメガ（Promega Corporation）はライフサイエンスの分野に生化学および分子生物学に関連する革新的で価値の高い製品を世界の研究者にお届けするリーディングカンパニーです。 • Production of ssDNA. The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing dual-reporter assays. Cloning - Promega pGEM-T Easy kit Ligation 1. A protocol for isolation of genomic DNA from white blood cells, tissue culture cells, animal and plant tissue, yeast, Gram-positive and Gram-negative bacteria. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid. Add to the reaction mixture: a. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. Seite nicht gefunden. Use the DNA molecular weight markers for both conventional and pulsed field gel electrophoresis applications. Bioz Stars score: 97/100, based on 2 PubMed citations. Bioz Stars score: 99/100, based on 177 PubMed citations. Promega AG beschäftigt 16 Mitarbeiter. Promega RNA purification products extract high-quality total RNA, mRNA or miRNA from many sample types, including blood, cells, tissues, FFPE tissue and total RNA preparations. GoTaq® Green Master Mix is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. Newton, C.R. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 16 PubMed citations. You are viewing: E1910 Change Configuration. 3 µl Fresh PCR product b. (1994) In: PCR, BIOS Scientific Publishers, Ltd., Oxford, UK, 13. These single-nucleotide overhangs anneal to the 3´‑thymidine overhangs of T-cloning vectors such as the Promega pGEM®-T (Cat.# A3600) and pGEM®-T Easy Vectors (Cat.# A1360) and the Invitrogen TOPO® TA Cloning Kit with pCR®2.1-TOPO® Vector (Invitrogen Cat.# K4500-01). pGEMt_vector - Free download as PDF File (.pdf), Text File (.txt) or read online for free. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 97/100, based on 572 PubMed citations. Company Promega; Price Supplier Page View Company Product Page; Catalog Number A3600; Quantity 20 reactions; Vector Name pGEM®-T Vector; Applications • Cloning PCR products. Many Promega Vectors are reporter vectors containing various combinations of luciferase genes (firefly, Renilla, NanoLuc luciferase) with other genetic elements. Pgemt, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 161 PubMed citations. ベクターマップ＆シークエンス Incubate at room temperature for 1 hour or overnight at 4°C 3. Bioz Stars score: 99/100, based on 16 PubMed citations. The luminescence signal from these reporter vectors is used as an indicator of the activity of any gene/DNA element fused to the reporter. Die Promega … DA: 59 PA: 43 MOZ Rank: 15. 5 µl 2x Rapid Ligation Buffer c. 1 µl pGEM-T vector d. 1 µl T4 DNA Ligase 2. The pGEM-T and pGEM-T Easy Systems now allow you to perform ligation reactions in as little as one hour. In this study the performance of the 2X Rapid Ligation Buffer is compared with that of the previously supplied T4 DNA Ligase 10X Buffer in both one-hour and 16-hour ligation reactions. On the basis of historical data, Reporter Gene Assay Kits market report provides key segments and their sub-segments, revenue and demand & supply data. Promega AG wurde in der Schweiz 1991 unter dem Namen Catalys AG gegründet. Falls Sie über einen defekten Link hierher gekommen sind, schreiben Sie bitte eine E-Mail an den Webmaster (webmaster@promega.cc).Falls es … Acids Res. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Store on ice until needed Transformation 1. プロトコル クイックプロトコル (pGEM-T Vectors) 製品マニュアル. The pGEM-T Easy vector has EcoRI restriction sites surrounding the proposed insert site, whereas the pGEM-T vector does not. Als Tochtergesellschaft von Promega Corp. bietet die Schweizer Niederlassung in Dübendorf zusätzlich und komplementär zu Produkten von Promega Corp. auch Labor- und Analysegeräte sowie Verbrauchsmaterialien der Firmen Serva GmbH an. Eukaryotische Expressionssysteme Analyse der Top-Unternehmen 2021 – Thermo Fisher, Agilent, New England Biolabs, Sigma Aldrich, Promega apexresearch März 9, 2021 Nischenmarktforschungsbericht 2021 über Eukaryotische Expressionssysteme Markt mit Branchenwachstumsanalyse, Wettbewerb, Trends und Prognose bis 2026. Theoretically, smaller inserts will be cloned more efficiently than longer inserts. Vector Pgemt Easy, supplied by Promega, used in various techniques. Read More About Sensitivity. The Global Reporter Gene Assay Kits market exhibits comprehensive information that is a valuable source of insightful data for business strategists during the decade 2014-2027. Cloning with pGEM®-T Vectors: Ligation; January 8, 2020; A Quick Method for A Tailing PCR Products; July 8, 2019; T-Vector Cloning: Questions, Answers and Tips ; November 12, 2019; The following two tabs change content below. • In vitro transcription from dual-opposed promoters. • Construction of unidirectional nested deletions with the Erase-a-Base® System. Protein expression vectors are basic tools for production of proteins. e-mail: office@promega.cc homepage: www.promega.cc Filmenbuchgericht: Feldkirch Filmenbuch: FN 336296d UST-IdNr. The pGEM-T and pGEM-T Easy plasmid vectors are essentially the same but with one important difference. Get cells out of freezer, thaw on ice for 5 minutes 2. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 97/100, based on 2 PubMed citations. Instructions for Use of Product(s) M7121: GoTaq Green Master Mix, M7122, M7123 Literature # 9PIM712. (1988) Nucl. The pGEM®-9Zf(–) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, and contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. The product portfolio includes RNA purification solutions for all sample throughput needs, from manual purification to high-throughput automated systems. Dual-Luciferase Reporter Assay and Dual-Luciferase Reporter 1000 Assay System Quick Protocol FB024 PDF (73 KB) – English. Technical Manual pGEM®-T and pGEM®-T Easy Vector Systems カタログカタログカタログ番号カタログ番号番号番号 A1360, A1380, A3600, A3610 www.promega.co.jp 注意注意注意：注意：：： この日本語マニュアルは製品に添付される英文マニュアルを翻訳したもの Page 4 Revised 5/07 GGAGA GCTCC CAACG CGTTG GATGC ATAGC TTGAG TATTC TATAG … What's in the box? Video Protocols. ATU65297145 ARA -Nummer: 17418 PROMEGA Handelsgesellschaft m.b.H Treietstraße 1 A-6833 Klaus Choose from a range of DNA, RNA and protein molecular weight markers. 製品マニュアル（日本語） DH5α使用説明書. pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual PDF (548 KB) – English. and Graham, A. www.promega.com Part# TM042 Printed in USA. E151A: 1 × 1 vial: Passive Lysis Buffer, 5X. Schweden. Complete Protocol. 16, 9677–86. Purified DNA is suitable for amplifications, restriction enzyme digestion and membrane hybridizations (e.g., Southern and dot/slot blots). Designed to work seamlessly with Promega assays, GloMax® microplate readers and luminometers come preloaded with protocols that allow you to run assays at the touch of a button. Robles, J. and Doers, M. (1994) Promega Notes 45, 19–20. Specifications. Protocols. Item Part # Size Available Separately; Luciferase Assay Substrate. Promega Technical Services Scientists are here to assist you in troubleshooting your experiments at any time.